Human Anti-CD3 Recombinant Antibody scFv Fragment (HPAB-M0467-YC-S(P))

Name: Human Anti-CD3 Recombinant Antibody scFv Fragment (HPAB-M0467-YC-S(P))
SKU: HPAB-M0467-YC-S(P)
Rating: 5 (1 reviews)

CAT#: HPAB-M0467-YC-S(P)

Provided is a monoclonal anti-CD3 antibody specific for the cluster of differentiation 3 (CD3). The antibody may be useful in the treatment of conditions such as prostate cancer.

Tested Data

SDS-PAGE

Figure 1 Human Anti-CD3 Recombinant Antibody (HPAB-M0467-YC-S(P)) in SDS-PAGE

SDS-PAGE analysis of HPAB-M0467-YC-S(P) in β-mercaptoethanol-reduced (Lane 1) and non-reduced (Lane 2) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced and non-reduced conditions, the purified proteins migrate as about 25-35 kDa which is consistent with theoretical size.

SEC-HPLC

Figure 2 Human Anti-CD3 Recombinant Antibody (HPAB-M0467-YC-S(P)) in SEC-HPLC

The purity of HPAB-M0467-YC-S(P) was analyzed by SEC-HPLC. The result shows that the scFv fragment forms a small number of dimers.

Column: 3 µm, 7.8 x 300 nm
Mobile phase: 150 mM Sodium Phosphate Buffer, pH 7.0
Detection: UV 280 nm
Injection: 10 µl

Figure 3 Human Anti-CD3 Recombinant Antibody (HPAB-M0467-YC-S(P)) in WB

Western blot analysis of HPAB-M0467-YC-S(P) was performed by loading human CD3 epsilon protein (Mouse IgG2a Fc Tag, 38.6 kDa) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for one hour. Membranes were probed with HPAB-M0467-YC-S(P) (2 ng/μL) and HRP mouse anti-His tag as a secondary antibody (1: 2000) in turn. Chemiluminescent detection was performed.

Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)

FACS

Figure 4 Human Anti-CD3 Recombinant Antibody (HPAB-M0467-YC-S(P)) in FACS

Flow cytometry analysis of Jurkat cells incubated with anti-human CD3 recombinant antibody cat# HPAB-M0467-YC-S(P). Jurkat cells were stained with HPAB-M0467-YC-S(P) (blue histogram, TE232382) or isotype control antibody (yellow histogram), followed by APC-conjugated goat anti-His tag secondary antibody. The used concentration of HPAB-M0467-YC-S(P) was 10 µg per 5 x 10^6 cells.

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Specifications

Host Species
Human

Derivation
Humanized

Type
Humanized scFv

Specificity
Human CD3

Species Reactivity
Human

Applications
FC

Product Property

Purity
>95% as determined by SDS-PAGE and HPLC analysis

Buffer
PBS

Preservative
No preservatives

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Alternative Names
Cluster of differentiation 3

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(Creative Biolabs Cat# HPAB-M0467-YC-S(P), RRID: AB_3111437)

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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - HPAB-M0467-YC-S(P).pdf

MSDS

MSDS - HPAB-M0467-YC-S(P).pdf

COA

Certificate of Analysis Lookup

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Figure 4 Human Anti-CD3 Recombinant Antibody (HPAB-M0467-YC-S(P)) in FACS

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